First comes the nipple.... then comes the butt!

Madonna continued stripping on her "MDNA Tour" as it landed in Rome, Italy on Tuesday this time flashing her rear.

While performing her hit "Human Nature" at the Stadio Olimpico, Madonna unzipped her pants and flashed her rear to the audience.

The pop queen was wearing black lace underwear and mesh tights. (WATCH VIDEO BELOW)

Recently she flashed her nipple at a concert in Istanbul, Turkey while performing the same song.

The video of Madonna showing her nipple went viral on YouTube and was highly commented with people mostly saying that she is too old to keep stripping.

Madonna also made headlines when she performed in Tel Aviv and showed an image of French National Front leader Marine Le Pen with a swastika.

The image was strongly criticized by the politician to the point that she threatened to sue Madonna if she shows it at her concert in France.

Watch Madonna Flashing Her Rear in Rome Below:

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Madonna