Justin Timberlake and Jessica Biel are now facing separation rumors after the singer was spotted without his wedding band on while his wife was notably absent for his recent Saturday Night Live appearance.

"Justin was solo again at the Saturday Night Live after party and he stayed until nearly 5 a.m.," a source told Radar Online. "Everyone was buzzing about her absence."

The site also noted that Biel, 31, was absent from Timberlake's American Music Awards performance in November, when she took to Twitter to say, "I'm watching from home tonight. Calm down, Internet."

But a source also revealed that Biel didn't accompany Timberlake, 32, to the show's rehersals on Friday.

"She wasn't with him at rehearsals on Friday either, which was sort of weird," the source said. "And it's not that Justin was flirting with other women or anything at the party but something just didn't seem right. It was literally what everyone was talking about; whether he and Jessica have split."

Back in October, tabloid magazine Star reported that the 32-year-old singer kissed Thalia, after performing at the 'Rock in Rio' Festival.

"It's no lie that Jessica is getting sick of all these rumors about her new husband. It's even more annoying because they're very much in love and are talking about starting a family next year. So for him to be linked to a woman who happens to be in the same picture is starting to wear a bit thin. That marriage is sound and anyone who says otherwise doesn't know them," the source said at the time.

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Justin Timberlake, Jessica Biel